Plasmid_Backbone
pSBBs1C

Part:BBa_K823023:Design

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-08-21)

pSBBs1C: Empty backbone for integration into Bacillus subtilis amyE locus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6084
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6084
    Illegal BamHI site found at 6066
    Illegal XhoI site found at 3574
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 6084
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6084
    Plasmid lacks a suffix.
    Illegal XbaI site found at 6099
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1128
    Illegal NgoMIV site found at 3538
    Illegal NgoMIV site found at 4677
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4501


Design Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR.


Source

This backbone is an igem compatible version of the Bacillus subtilis vector pDG1662

References

[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]